Separation of Deciduomal Cells by Velocity Sedimentation at Unit Gravity

Isolation of rat gonocytes by velocity sedimentation at unit gravity.

A method for obtaining enriched populations of gonocytes from rat embryos at 18 days p.c. has been developed. Single cell suspensions with high cell yield and good viability of the cells were obtained by a collagenase/trypsin digestion of the testes. Cells were separated on the basis of size by the Staput technique of velocity sedimentation at unit gravity. Populations of 600,000 gonocytes (70-...

Separation of erythropoietin-sensitive cells from hemopoietic spleen colony-forming stem cells of mouse fetal liver by unit gravity sedimentation.

Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation

Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, w...

Separation of immunocompetent cells from human and mouse hemopoietic cell suspensions by velocity sedimentation.

Separation of the erythropoietin-responsive progenitors BFU-E and CFU-E in mouse bone marrow by unit gravity sedimentation.

The sedimentation velocity profiles of the entities in mouse bone marrow responsible for erythropoietic burst formation (BFU-E) and for erythrocytic colony formation (CFU-E) have been studied under conditions designed to determine whether the values observed are real or result from cell interactions occurring during culture of the fractions. Bone marrow cells of adult C3Hf/Bi mice were subjecte...